Description:

VMP1 is a stress-induced protein that, when overexpressed, promotes formation of intracellular vacuoles followed by cell death. May be involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis.VMP1 is a transmembrane protein that co-localizes with LC3, a marker of the autophagosomes. It interacts with beclin-1, a mammalian autophagy initiator, through the VMP1-Atg domain, which is essential for autophagosome formation. VMP1 endogenous expression co-localizes with LC3 in pancreas tissue undergoing pancreatitis-induced autophagy.

Product Specification:

Alternative Name:	VMP1, TMEM49
Host:	Rabbit
Immunogen:	Synthetic peptide corresponding to aa 386-406 of human vacuole membrane protein 1 (VMP1).
UniProt ID:	Q96GC9
Species reactivity:	Human, Mouse, Rat
Crossreactivity:	Ovalbumin
Applications:	ICC, IP, WB
Recommended Dilutions/Conditions:	Immunocytochemistry (1:200) Immunoprecipitation (5µg/mg lysate) Western Blot (1:100-1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Formulation:	Liquid. Purified serum containing 0.2% sodium azide.
Handling:	Avoid freeze/thaw cycles.
Shipping:	Shipped on Blue Ice
Long Term Storage:	-20°C
Scientific Background:	VMP1 is a transmembrane protein that co-localizes with LC3, a marker of the autophagosomes. It interacts with beclin-1, a mammalian autophagy initiator, through the VMP1-Atg domain, which is essential for autophagosome formation. VMP1 endogenous expression co-localizes with LC3 in pancreas tissue undergoing pancreatitis-induced autophagy.
Protocol:	Standard Procedure for Immunocytochemistry Grow cells on glass coverslips. Rinse briefly in phosphate-buffered saline (PBS). Fix cells with 4% paraformaldehyde in PBS, pH 7.4 for 10 min at room temperature. Wash cells three times with PBS. Incubate the samples for 5 min with PBS containing 0.1% Triton X-100. Wash 3 x 5 min with PBS. Incubate cells with 1% BSA in PBS for 1 hour at room temperature (this time can be adjusted to the study system). Incubate with primary antibody diluted in PBS containing 1% BSA in a humidified chamber for 1hr at room temperature. VMP1 antibody dilution 1:200. Wash 1 x 5 min with 0.05% Tween-20 in PBS. Wash 2 x 5 min with PBS. Incubate with secondary antibody in PBS containing 1% BSA for 1 h at room temperature in the dark. Alexa Fluor conjugated antibodies dilution 1:1000. Wash 2 x 5 min with 0.05% Tween-20 in PBS. Wash 3 x 5 min with PBS. (OPTIONAL) Incubate cells with DAPI (DNA stain) for 1 min. Rinse with PBS. Mount coverslip with a drop of mounting medium. Seal coverslip with nail polish to prevent drying and analyze under a microscope. Store coverslips in the dark at -20°C.

Product categoriesPharmaceuticals And Biochemic CAS 6090-98-8 conduritol F CAS 20544-37-0 2,4,8,10-Tetraoxa-3, CAS 135564-23-7 Benzaldehyde, 4-amin CAS 34169-62-5 2-Naphthalenesulfoni CAS 6305-52-8 2-Butyldecahydronaph CAS 10074-13-2 1,3-Benzenedimethane CAS 84434-05-9 Acetic acid,2-[(9-ox CAS 104748-51-8 Avilamycin K CAS 5539-08-2 Ergoline-8-carboxyli CAS 1242-56-4 Androst-1-en-3-one,1 CAS 52978-30-0 (1R,2R,3E,5E,15E,17E CAS 24471-48-5 2-Pyrenemethanol CAS 39325-01-4 Picibanil CAS 244290-68-4 Quinolinium, 7-[(die CAS 80584-90-3 1H-Benzotriazole-1-m CAS 65193-87-5 Butanoic acid, 3-oxo CAS 3563-92-6 Zylofuramine CAS 24979-74-6 Phenol,4-ethenyl-, p CAS 1067-48-7 1-Pentanamine, 5-(tr CAS 62886-59-3 Sephadex-CM C-25,ion View all products Company Profile Name:Enzo Life SciencesCategory:Corporation/Limited Liability CompanyTrade Category: ManufacturerRegistered Time: 1996Registered Capital: Above US$100 MillionLegal Representative:Employees:Address: TaksimWebsite: http://www.enzolifesciences.comProduct/Service: chemicals Our breadth of scientific expertise coupled with unique technology platforms give us the flexibility to drive innovation throughout the entire research continuum. From life sciences research discovery efforts, through drug development, and into clinical research, our comprehensive range of products are designed to support your goals. When a standard catalog product does not meet your needs, talk to us about custom solutions; we can help