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当前位置: 首页 > 产品中心 > exosome_research > 酶学/蛋白酶体20S和β;2亚单位单克隆抗体(MCP165)/BML-PW9300-0100/100µ;L
商品详细酶学/蛋白酶体20S和β;2亚单位单克隆抗体(MCP165)/BML-PW9300-0100/100µ;L
酶学/蛋白酶体20S和β;2亚单位单克隆抗体(MCP165)/BML-PW9300-0100/100µ;L
酶学/蛋白酶体20S和β;2亚单位单克隆抗体(MCP165)/BML-PW9300-0100/100µ;L
商品编号: BML-PW9300-0100
品牌: enzolifesciences
市场价: ¥7020.00
美元价: 4212.00
产地: 美国(厂家直采)
公司:
产品分类: 外泌体研究
公司分类: exosome_research
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Product Specification:

Alternative Name:Proteasome subunit β type-7, Macropain chain Z
 
Clone:MCP165
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Dinitrophenylated proteasomes.
 
UniProt ID:Q99436 (human), P70195 (mouse)
 
Gene/Protein Identifier:PSMB7 (gene name)
 
Species reactivity:Human, Mouse
 
Specificity:Recognizes the β2 subunit of the 20S proteasome.
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Immunoprecipitation: Does not immunoprecipitate intact proteasomes from HeLa cell preparations.
Immunohistochemistry: Has been shown to have applicability in immunohistochemical applications using human tissue.
Western blot: Single dimension SDS-PAGE of a HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of ~28kDa.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Use/Stability:Store unopened vial at -20°C until required for use. Aliquot undiluted antibody into smaller volumes (not less than 10μl) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The hybridoma secreting the antibody to subunit β2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunization with dinitrophenylated proteasomes. The antibody (clone MCP165) has been characterised by one- and two dimensional Western blotting. The antibody has not been demonstrated to immunoprecipitate the native 20S proteasome. The immunoglobulin subclass is IgG1.
 
Technical Info/Product Notes:Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.
 
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