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Enzolifesciences/Proteasome 20S β2 subunit monoclonal antibody (MCP165)/BML-PW9300-0025/25µl
Enzolifesciences/Proteasome 20S β2 subunit monoclonal antibody (MCP165)/BML-PW9300-0025/25µl
商品编号:
BML-PW9300-0025
品牌:
enzolifesciences
市场价:
¥2260.00
美元价:
1356.00
产品分类:
外泌体研究
公司分类:
exosome_research
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Product Specification:
Alternative Name: | Proteasome subunit β type-7, Macropain chain Z |
Clone: | MCP165 |
Host: | Mouse |
Isotype: | IgG1 |
Immunogen: | Dinitrophenylated proteasomes. |
UniProt ID: | Q99436 (human), P70195 (mouse) |
Gene/Protein Identifier: | PSMB7 (gene name) |
Species reactivity: | Human, Mouse |
Specificity: | Recognizes the β2 subunit of the 20S proteasome. |
Applications: | IHC, WB |
Recommended Dilutions/Conditions: | Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
Application Notes: | Immunoprecipitation: Does not immunoprecipitate intact proteasomes from HeLa cell preparations. Immunohistochemistry: Has been shown to have applicability in immunohistochemical applications using human tissue. Western blot: Single dimension SDS-PAGE of a HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of ~28kDa. |
Purity Detail: | Protein G affinity purified. |
Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
Use/Stability: | Store unopened vial at -20°C until required for use. Aliquot undiluted antibody into smaller volumes (not less than 10μl) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. |
Handling: | Avoid freeze/thaw cycles. |
Shipping: | Shipped on Blue Ice |
Long Term Storage: | -20°C |
Scientific Background: | The hybridoma secreting the antibody to subunit β2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunization with dinitrophenylated proteasomes. The antibody (clone MCP165) has been characterised by one- and two dimensional Western blotting. The antibody has not been demonstrated to immunoprecipitate the native 20S proteasome. The immunoglobulin subclass is IgG1. |
Technical Info/Product Notes: | Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins. |
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Company Profile
Name:Enzo Life SciencesCategory:Corporation/Limited Liability CompanyTrade Category: ManufacturerRegistered Time: 1996Registered Capital: Above US$100 MillionLegal Representative:Employees:Address: TaksimWebsite: http://www.enzolifesciences.comProduct/Service: chemicals
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