酶科学/P-糖蛋白单克隆抗体（C219）/ALX-801-002-C100/1ml
商品编号：
ALX-801-002-C100
品牌：
enzolifesciences
市场价：
¥15460.00
美元价：
9276.00
产品分类：
外泌体研究
公司分类：
exosome_research
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Product Specification:
| Alternative Name: | ABCB1, CD243, P-glycoprotein 1 |
| Clone: | C219 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | SDS-solubilized plasma membranes of an MDR (multidrug resistant) Chinese hamster ovary (CHO) cell line and a human cell line. |
| UniProt ID: | P08183 |
| Species reactivity: | Human, Mouse, Rat Dog, Primate |
| Specificity: | Recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein. |
| Crossreactivity: | Cross-reacts with a ~200kDa protein which migrates in the same position as myosin and with C-erbB2 protein (p185c-erbB2). |
| Applications: | ICC, IHC (FS), IHC (PS), IP |
| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections): - For optimal staining, the sections should be pretreated with an antigen unmasking solution. - The antibody may be diluted to ≥1:25 for biotin based detection systems. - For optimal staining, the primary antibody should be incubated 60 minutes at room temperature. Western Blot (1:50-1:100) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Flow Cytometry: cell permeabilization required. Detects bands of ~170kDa (MDR1 P-glycoprotein) and of ~140kDa (MDR3 P-glycoprotein) by Western blot. Cited applications include ELISA, IF, FC, and WB |
| Positive Control: | Cell lines: Appropriate drug-sensitive parental cell lines and their multidrug resistant derivatives prepared by the same methods as the test specimens can be used as control materials. Tissue: Human liver (positive staining detected along luminal surfaces of bile canaliculi) or human colon (positive staining localized to luminal surface of secretory epithelium) are recommended. |
| Formulation: | Liquid. In 0.01M PBS containing 1% BSA and 0.1% sodium azide. |
| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
| Shipping: | Shipped on Blue Ice |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
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Company Profile
Name:Enzo Life SciencesCategory:Corporation/Limited Liability CompanyTrade Category: ManufacturerRegistered Time: 1996Registered Capital: Above US$100 MillionLegal Representative:Employees:Address: TaksimWebsite: http://www.enzolifesciences.comProduct/Service: chemicals
Our breadth of scientific expertise coupled with unique technology platforms give us the flexibility to drive innovation throughout the entire research continuum. From life sciences research discovery efforts, through drug development, and into clinical research, our comprehensive range of products are designed to support your goals. When a standard catalog product does not meet your needs, talk to us about custom solutions; we can help
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