酶科学/LC3单克隆抗体（5H3）/ALX-803-082-C100/100µ；G
商品编号：
ALX-803-082-C100
品牌：
enzolifesciences
市场价：
¥11100.00
美元价：
6660.00
产品分类：
外泌体研究
公司分类：
exosome_research
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Product Specification:
| Alternative Name: | Microtubule-associated protein 1 light chain 3, MAP1LC3 |
| Clone: | 5H3 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to an internal sequence identical in LC3A, LC3B and LC3C conjugated to hemocyanin. |
| UniProt ID: | Q9H492 (LC3A), Q9GZQ8 (LC3B), Q9BXW4 (LC3C) |
| Species reactivity: | Human |
| Specificity: | Recognizes both forms of endogenous LC3. |
| Applications: | ICC, IHC, WB |
| Recommended Dilutions/Conditions: | Immunocytochemistry (paraformaldehyde/methanol fixation; 1-10 µg/ml) Western Blot (0.5µg/ml for HRPO/ECL detection; Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) We strongly recommend to use PVDF membranes for immunoblot assays. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects a band of ~18kDa (LC3-I; cytoplasmic form) and a band of ~16kDa (LC3-II; lipidated form) by Western blot. |
| Positive Control: | Included. (Prod. No. ALX-840-038) |
| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
| Formulation: | Liquid. In PBS containing 50% glycerol, 0.09% sodium azide, PEG and sucrose. |
| Use/Stability: | Stable at -20°C up to 1 year. |
| Handling: | Thawed aliquots may be stored at 4°C up to 3 months. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -80°C |
| Scientific Background: | Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings. |
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Company Profile
Name:Enzo Life SciencesCategory:Corporation/Limited Liability CompanyTrade Category: ManufacturerRegistered Time: 1996Registered Capital: Above US$100 MillionLegal Representative:Employees:Address: TaksimWebsite: http://www.enzolifesciences.comProduct/Service: chemicals
Our breadth of scientific expertise coupled with unique technology platforms give us the flexibility to drive innovation throughout the entire research continuum. From life sciences research discovery efforts, through drug development, and into clinical research, our comprehensive range of products are designed to support your goals. When a standard catalog product does not meet your needs, talk to us about custom solutions; we can help
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