Description:

A frequent cause of high background staining is due to non-specific binding of secondary antibodies. High quality secondary antibodies are crucial to obtaining good staining of targets that are normally harder to detect, for example due to low expression levels. Interference caused by endogenous immunoglobulins can also result in poor detection. Secondary antibodies specifically adsorbed against cross-reacting immunoglobulins will enhance results. ALX-211-502 is recommended for use with select ALEXIS recombinant fusion proteins containing the Fc portion of human IgG.

Product Specification:

Host:	Goat
Immunogen:	Human IgG.
Species reactivity:	Human
Specificity:	Recognizes the heavy chain of human IgG.
Applications:	ELISA, Flow Cytometry, ICC, IHC (FS), IHC (PS), WB
Application Notes:	Use with a streptavidin detection system.
Purity Detail:	Purified by affinity chromatography on human IgG covalently linked to agarose. Cross-adsorbed with human IgM, IgD and IgA. Cross-reactivity with IgG from other species has not been evaluated.
Formulation:	Liquid. In PBS, pH 7.4, containing 0.1% sodium azide.
Use/Stability:	For increased long-term stability, add 40% glycerol to aliquots prior to freezing at -20°C.
Handling:	Protect from light. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:	Shipped on Blue Ice
Short Term Storage:	+4°C
Long Term Storage:	-20°C

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