Description:

Specificity: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human, mouse, bovine, horse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.

Product Specification:

Host:	Goat
Immunogen:	Purified rat IgG (whole molecule).
Species reactivity:	Rat
Specificity:	Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Applications:	ELISA, ICC, IHC, WB
Recommended Dilutions/Conditions:	ELISA (1:5,000-1:100,000) Immunocytochemistry (1:500-1:5,000) Immunohistochemistry(1:500-1:5,000) Western Blot (1:10,000-1:200,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Purity Detail:	Immunoaffinity purified.
Quality Control:	The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human, mouse, bovine, horse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.
Formulation:	Liquid. In 0.005M sodium phosphate, pH 7.6, containing 0.125M sodium chloride, 7.5mg/ml BSA (IgG-free, protease-free), and 50% glycerol.
Use/Stability:	Warning that sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Handling:	Avoid freeze/thaw cycles.
Shipping:	Shipped on Blue Ice
Long Term Storage:	-20°C
Scientific Background:	Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains.  Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain.

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