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Enzolifesciences/Glutathione S-transferase-Pi monoclonal antibody (USal-hGST-Pi-McAb-1)/ALX-804-510-C100/100µg
Product Specification:
Alternative Name: | GST-π, GST-P, Glutathione S-transferase P, GSTP1 |
Clone: | USal-hGST-Pi-McAb-1 |
Host: | Mouse |
Isotype: | IgG1 |
Immunogen: | Recombinant human placenta GST-Pi (glutathione S-transferase-Pi). |
UniProt ID: | P09211 |
Species reactivity: | Human, Rat Leishmania |
Crossreactivity: | Does not cross-react with human or rat class alpha GST. |
Applications: | IHC (FS), WB |
Recommended Dilutions/Conditions: | Immunohistochemistry (frozen sections, 1:100) Western Blot (1:150-1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
Positive Control: | Rat hepatocarcinoma can be used as positive control. |
Purity Detail: | Protein G-affinity purified. |
Formulation: | Liquid. Contains 100nM sodium azide and 0.7% BSA. |
Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
Shipping: | Shipped on Blue Ice |
Short Term Storage: | +4°C |
Long Term Storage: | -20°C |
Scientific Background: | The GST Family Glutathione S-transferases (GSTs) are a large multigen family of multifunctional enzymes, which play an important role in detoxification of potentially genotoxic chemicals, by catalyzing the conjugation of glutathione to a large number of hydrophobic and electrophilic compounds, including carcinogens. They also bind to a variety of nonsubstrate ligands. The GSTs are widely distributed in mammalian species and can be grouped into four classes, based on their biochemical, immunological and structural properties: alpha, mu, pi and theta. GSTs are dimeric, mainly cytosolic, enzymes that have extensive ligand binding properties in addition to their catalytic role in detoxification. A separate microsomal class of GSTs exists which is quite distinct from the cytosolic GSTs, and is designated as "membrane-associated proteins in eicosanoid and glutathione metabolism" (MAPEG). The N-terminal region tends to be better conserved within classes as it includes an important part of the active site. GSTs are believed to play a role in cancer prevention, as the electrophilic compounds that are conjugated to glutathione could otherwise react with and modify cellular components such as DNA, thereby initiating carcinogenesis. On the other hand, GSTs might detoxify chemotherapeutic drugs in cancer cells, thus contributing to drug resistance. |