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当前位置: 首页 > 产品中心 > exosome_research > Enzolifesciences/GluR1 (extracellular) polyclonal antibody/BML-SA612-0200/200µl
商品详细Enzolifesciences/GluR1 (extracellular) polyclonal antibody/BML-SA612-0200/200µl
Enzolifesciences/GluR1 (extracellular) polyclonal antibody/BML-SA612-0200/200µl
Enzolifesciences/GluR1 (extracellular) polyclonal antibody/BML-SA612-0200/200µl
商品编号: BML-SA612-0200
品牌: enzolifesciences
市场价: ¥11100.00
美元价: 6660.00
产地: 美国(厂家直采)
公司:
产品分类: 外泌体研究
公司分类: exosome_research
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Product Specification:

Alternative Name:Glutamate receptor 1, AMPA-selective glutamate receptor 1, Glutamate receptor ionontropic AMPA 1
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 271-285 of rat GluR1 (GRIA1).
 
UniProt ID:P19490
 
Species reactivity:Mouse, Rat
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:100)
Western Blot (1:200)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Blocking Peptide:Included: GluR1 (extracellular) Control Peptide (100µg). Supplied lyophilized.
 
Purity Detail:Affinity purified
 
Formulation:Lyophilized from PBS, pH 7.4, 1% BSA and 0.025% sodium azide.
 
Reconstitution:To lyophilized antibody, add 50µl or 200µl (depending on which size was purchased) of deionized water. Further dilutions should be made using a carrier protein such as 1% BSA. To lyophilized control peptide, add 100µl of distilled water.
 
Use/Stability:Lyophilized antibody and lyophilized peptide can be stored intact at room temperature for several weeks. For longer periods they should be stored at -20°C. After reconstitution, the antibody solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.Centrifuge all antibody preparations before use (10,000 X g for 5 minutes). After reconstitution, control peptide should be stored at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:GluR receptors are permeable to cations Na and K and Ca2+. Gating of Glu receptors by glutamate is extremely fast and therefore the Glu receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity.
 
Technical Info/Product Notes:PREADSORPTION CONTROL: 1µg control peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments.
 
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