Description:

Widely described antibody against α-galactose residues with broad cross-reactivity among different species. Useful for measuring the α-Gal epitope expression on cells, glycolipids and glycoproteins, characterization of hyperacute rejection (HAR) in organ and tissue transplantations and monitoring xenotransplantation experiments. The IgM isotype mimicks in vivo reactions and can be used for cytotoxicity assays for α-Gal specific pathways (addition or presence of complement necessary). Ideal as a high-throughput screening tool for inhibitors of antibody induced cytotoxicity.
Organ transplantation from pig to human result in HAR. Humans naturally produce large quantities of anti-α-Gal antibodies, which represent 1-3% of all circulating immunoglobulins and are produced by about 1% of all B cells. When pig organs or tissues are transplanted into the human body, the IgM isotype of anti-Gal binds to α-Gal epitopes, which causes activation of the complement cascade, resulting in cell lysis. This rapid activation of complement by anti-Gal IgM is an immunological barrier that poses the greatest risk of initiating HAR. The monoclonal antibody M86 to Galα1-3Gal epitopes developed by U. Galili, et al. is an essential tool in the study of human xenotransplantation and related research.

Product Specification:

Alternative Name:	α-Galactose 1,3
Clone:	M86
Host:	Mouse
Isotype:	IgM
Immunogen:	Rabbit red blood cell membrane.
Source:	Hybridoma tissue culture.
Species reactivity:	Mouse, Rat Porcine
Specificity:	Recognizes synthetic and naturally produced mouse, rat and pig Galα1-3Gal epitopes on glycoproteins and glycolipids.
Crossreactivity:	Does not cross-react with β-Gal glycoproteins or BSA.
Applications:	ELISA, Flow Cytometry, IHC (PS), WB, FUNC
Recommended Dilutions/Conditions:	Note: Do not use non-fat milk for blocking! Many blocking agents from non-human mammal sources will cross-react with the antibody. Appropriate antibody dilution may vary from lot to lot. An initial dilution of 1:5 is recommended. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:	Functional Application: can be used for cytotoxicity assays for α-Gal specific pathways
Quantity:	4 mL per vial
Formulation:	Liquid. Tissue culture supernatant.
Use/Stability:	Stable for at least 3 years when stored at -20°C.
Handling:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:	Shipped on Dry Ice
Long Term Storage:	-20°C

Product categoriesPharmaceuticals And Biochemic CAS 6090-98-8 conduritol F CAS 20544-37-0 2,4,8,10-Tetraoxa-3, CAS 135564-23-7 Benzaldehyde, 4-amin CAS 34169-62-5 2-Naphthalenesulfoni CAS 6305-52-8 2-Butyldecahydronaph CAS 10074-13-2 1,3-Benzenedimethane CAS 84434-05-9 Acetic acid,2-[(9-ox CAS 104748-51-8 Avilamycin K CAS 5539-08-2 Ergoline-8-carboxyli CAS 1242-56-4 Androst-1-en-3-one,1 CAS 52978-30-0 (1R,2R,3E,5E,15E,17E CAS 24471-48-5 2-Pyrenemethanol CAS 39325-01-4 Picibanil CAS 244290-68-4 Quinolinium, 7-[(die CAS 80584-90-3 1H-Benzotriazole-1-m CAS 65193-87-5 Butanoic acid, 3-oxo CAS 3563-92-6 Zylofuramine CAS 24979-74-6 Phenol,4-ethenyl-, p CAS 1067-48-7 1-Pentanamine, 5-(tr CAS 62886-59-3 Sephadex-CM C-25,ion View all products Company Profile Name:Enzo Life SciencesCategory:Corporation/Limited Liability CompanyTrade Category: ManufacturerRegistered Time: 1996Registered Capital: Above US$100 MillionLegal Representative:Employees:Address: TaksimWebsite: http://www.enzolifesciences.comProduct/Service: chemicals Our breadth of scientific expertise coupled with unique technology platforms give us the flexibility to drive innovation throughout the entire research continuum. From life sciences research discovery efforts, through drug development, and into clinical research, our comprehensive range of products are designed to support your goals. When a standard catalog product does not meet your needs, talk to us about custom solutions; we can help