Description:

Oxidative stress resulting in the formation of biological radicals has been implicated in a number of human diseases, such as cancer and aging. There is, however, a paucity of reliable methods for in vivo or ex vivo detection of radical formation. Until now the only general technique that allowed for the detection of these highly reactive species was electron spin resonance (ESR) using spin traps. One of the most popular of these spin traps is 5,5-dimethyl-1-pyrroline N-oxide (DMPO). In the ESR method, radicals are trapped by DMPO, and the DMPO spin adduct signal is measured quantitatively by an ESR spectrometer. The DMPO, mAb (1) (Prod. No. ALX-803-340) specifically reacts with DMPO-protein and DMPO-DNA adducts and therefore highly simplifies the detection of radical formation compared to the ESR method.

Product Specification:

Alternative Name:	5,5-dimethyl-1-pyrroline-N-oxide
Clone:	1
Host:	Mouse
Isotype:	IgG
Immunogen:	DMPO-octanoic acid conjugated to ovalbumin.
Source:	Hybridoma tissue culture supernatant.
Species reactivity:	Species independent
Specificity:	Recognizes DMPO (Prod. No. ALX-430-090), DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts.
Crossreactivity:	Does not cross-react with non-adducted proteins or DNA. Does not cross-react with ovalbumin.
Applications:	ELISA, IP, WB
Recommended Dilutions/Conditions:	Western Blot (1-10ug/ml) ELISA (1-10ug/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Purity Detail:	Protein G-affinity purified.
Formulation:	Liquid. In PBS, pH 7.4, containing 10% glycerol and 0.02% sodium azide.
Handling:	Dilute only prior to immediate use.
Shipping:	Shipped on Blue Ice
Long Term Storage:	+4°C

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