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Enzolifesciences/C-erbB-2 (human) clonal antibody (A24-V)/ALX-810-227-L001/1ml

Enzolifesciences/C-erbB-2 (human) clonal antibody (A24-V)/ALX-810-227-L001/1ml
商品编号:
ALX-810-227-L001
品牌:
enzolifesciences
市场价:
¥20200.00
美元价:
12120.00
产品分类:
外泌体研究
公司分类:
exosome_research
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
ProductSpecification:
AlternativeName: | ErbB2,HER-2,NEUproto-oncogene |
Clone: | A24-V |
Host: | Rabbit |
Isotype: | IgG |
Immunogen: | SyntheticpeptidecorrespondingtotheC-terminalregionofhumanC-erbB-2. |
UniProtID: | P04626 |
Speciesreactivity: | Human |
Applications: | IHC(FS),IHC(PS) |
RecommendedDilutions/Conditions: | Immunohistochemistry(paraffinsections,1:100-1:300) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
PurityDetail: | MajorcloneofIgGsobtainedfromimmunoaffinitypurifiedimmunoglobulinscorrespondingtoimmunogenicpeptide. |
Formulation: | Liquid.In20mMTRIS/HCl,pH8.0,containing20mg/mlBSAand0.05%sodiumazide. |
Use/StABIlity: | Stableforatleast12monthsafterreceiptwhenstoredasrecommended. |
Handling: | Afteropening,preparealiquotsandstoreat+4°C. |
Shipping: | BlueIceNotFrozen |
LongTermStorage: | +4°C |
TechnicalInfo/ProductNotes: | ClonalRabbitAntibodyisapurehomogenousfractionofrabbitimmunoglobulin(IgG)whichcorrespondstoauniquelinearepitopeonthetargetprotein.Thisepitopeisdesignedafteradetailedanalysisoftheantigenstructure,regulatorypost-translationalmodifications,protein/proteininteractiondomainandproteinfolding.Thechosensequence(peptide)issynthesizedandtherabbitisimmunized.Thecloneisthenseparatedfromthecrudeantiserumby"invitrocloningtechnology".Theresultingproductismonospecificandcharacterizedbysuperiorspecificity,affinityandAvidity. |
Protocol: | IHC(paraffinsections)Protocol: 1.Deparaffinizethesectionin3changesofxylene,5minuteseach. 2.Washthesectionin96%,80%and70%benzylalcoholfor5minuteseach. 3.Rinseindistilledwater. 4.Blocktheendogenousperoxidasebyincubatingthetissuein3%hydrogenperoxide(H2O2)for10minutes. 5.Washindistilledwater. 6.Forantigenretrievaluseoneofthefollowingprocedures: A)ImmersetheslideinTRIS-EDTAbuffer,pH9.0,0.05%Tween-20*,andincubateinmicrowave(600W)for10minutes,or B)Immersetheslideincitratebuffer,pH6.0,0.05%Tween-20**,andincubateinmicrowave(600W)for10minutes. 7.Removethestainingtoroomtemperatureandlettheslidetocoolfor15minutes. 8.Rinseindistilledwater. 9.Washin0.05MTRIS-HCl,pH7.6buffersupplementedwith0.5%ofTween-20(bufferA)for5minutes. 10.IncubatethesectionwithprimaryantibodydilutedinbufferAatthedilution1:100-1:300for1hourintheclosedwetchamber. 11.Washtwice5minuteswithbufferA. 12.Applythesecondaryantibody(theprotocoldependsonthesupplier),andproceedtostandardimmunohistochemistryprotocol(HRP-Peroxide-DAB). 13.Washtwice5minuteswithbufferA. 14.Applyofchromogen(DAB),10minutes. 15.Washinwater-10minutes. 16.Staininhematoxylinfor5minutes. 17.Washinwater-10minutes. 18.Dehydratethesectionin2changesof96%benzylalcoholfor5minuteseach. 19.Washthesectionin2changesofxylenefor2minuteseach. 20.Mounttheslideforobservation. *TRIS-EDTABuffer(10mMTRISBase,1mMEDTAsolution,0.05%Tween-20,pH9.0): **CitrateBuffer(10mMCitricAcid,0.05%Tween-20,pH6.0): |