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商品详细Enzolifesciences/C-erbB-2 (human) clonal antibody (A24-V)/ALX-810-227-L001/1ml
Enzolifesciences/C-erbB-2 (human) clonal antibody (A24-V)/ALX-810-227-L001/1ml
Enzolifesciences/C-erbB-2 (human) clonal antibody (A24-V)/ALX-810-227-L001/1ml
商品编号： ALX-810-227-L001
品牌： enzolifesciences
市场价： ¥20200.00
美元价： 12120.00
产地： 美国(厂家直采)
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产品分类： 外泌体研究
公司分类： exosome_research
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Product categoriesPharmaceuticals And Biochemic CAS 6090-98-8 conduritol F CAS 20544-37-0 2,4,8,10-Tetraoxa-3, CAS 135564-23-7 Benzaldehyde, 4-amin CAS 34169-62-5 2-Naphthalenesulfoni CAS 6305-52-8 2-Butyldecahydronaph CAS 10074-13-2 1,3-Benzenedimethane CAS 84434-05-9 Acetic acid,2-[(9-ox CAS 104748-51-8 Avilamycin K CAS 5539-08-2 Ergoline-8-carboxyli CAS 1242-56-4 Androst-1-en-3-one,1 CAS 52978-30-0 (1R,2R,3E,5E,15E,17E CAS 24471-48-5 2-Pyrenemethanol CAS 39325-01-4 Picibanil CAS 244290-68-4 Quinolinium, 7-[(die CAS 80584-90-3 1H-Benzotriazole-1-m CAS 65193-87-5 Butanoic acid, 3-oxo CAS 3563-92-6 Zylofuramine CAS 24979-74-6 Phenol,4-ethenyl-, p CAS 1067-48-7 1-Pentanamine, 5-(tr CAS 62886-59-3 Sephadex-CM C-25,ion View all products Company Profile Name:Enzo Life SciencesCategory:Corporation/Limited Liability CompanyTrade Category: ManufacturerRegistered Time: 1996Registered Capital: Above US$100 MillionLegal Representative:Employees:Address: TaksimWebsite: http://www.enzolifesciences.comProduct/Service: chemicals Our breadth of scientific expertise coupled with unique technology platforms give us the flexibility to drive innovation throughout the entire research continuum. From life sciences research discovery efforts, through drug development, and into clinical research, our comprehensive range of products are designed to support your goals. When a standard catalog product does not meet your needs, talk to us about custom solutions; we can help
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AlternativeName:ErbB2,HER-2,NEUproto-oncogene
 
Clone:A24-V
 
Host:Rabbit
 
Isotype:IgG
 
Immunogen:SyntheticpeptidecorrespondingtotheC-terminalregionofhumanC-erbB-2.
 
UniProtID:P04626
 
Speciesreactivity:Human
 
Applications:IHC(FS),IHC(PS)
 
RecommendedDilutions/Conditions:Immunohistochemistry(paraffinsections,1:100-1:300)
Suggesteddilutions/conditionsmaynotbeavailableforallapplications.
Optimalconditionsmustbedeterminedindividuallyforeachapplication.
 
PurityDetail:MajorcloneofIgGsobtainedfromimmunoaffinitypurifiedimmunoglobulinscorrespondingtoimmunogenicpeptide.
 
Formulation:Liquid.In20mMTRIS/HCl,pH8.0,containing20mg/mlBSAand0.05%sodiumazide.
 
Use/StABIlity:Stableforatleast12monthsafterreceiptwhenstoredasrecommended.
 
Handling:Afteropening,preparealiquotsandstoreat+4°C.
 
Shipping:BlueIceNotFrozen
 
LongTermStorage:+4°C
 
TechnicalInfo/ProductNotes:ClonalRabbitAntibodyisapurehomogenousfractionofrabbitimmunoglobulin(IgG)whichcorrespondstoauniquelinearepitopeonthetargetprotein.Thisepitopeisdesignedafteradetailedanalysisoftheantigenstructure,regulatorypost-translationalmodifications,protein/proteininteractiondomainandproteinfolding.Thechosensequence(peptide)issynthesizedandtherabbitisimmunized.Thecloneisthenseparatedfromthecrudeantiserumby"invitrocloningtechnology".Theresultingproductismonospecificandcharacterizedbysuperiorspecificity,affinityandAvidity.
 
Protocol:IHC(paraffinsections)Protocol:
1.Deparaffinizethesectionin3changesofxylene,5minuteseach.
2.Washthesectionin96%,80%and70%benzylalcoholfor5minuteseach.
3.Rinseindistilledwater.
4.Blocktheendogenousperoxidasebyincubatingthetissuein3%hydrogenperoxide(H2O2)for10minutes.
5.Washindistilledwater.
6.Forantigenretrievaluseoneofthefollowingprocedures:
A)ImmersetheslideinTRIS-EDTAbuffer,pH9.0,0.05%Tween-20*,andincubateinmicrowave(600W)for10minutes,or
B)Immersetheslideincitratebuffer,pH6.0,0.05%Tween-20**,andincubateinmicrowave(600W)for10minutes.
7.Removethestainingtoroomtemperatureandlettheslidetocoolfor15minutes.
8.Rinseindistilledwater.
9.Washin0.05MTRIS-HCl,pH7.6buffersupplementedwith0.5%ofTween-20(bufferA)for5minutes.
10.IncubatethesectionwithprimaryantibodydilutedinbufferAatthedilution1:100-1:300for1hourintheclosedwetchamber.
11.Washtwice5minuteswithbufferA.
12.Applythesecondaryantibody(theprotocoldependsonthesupplier),andproceedtostandardimmunohistochemistryprotocol(HRP-Peroxide-DAB).
13.Washtwice5minuteswithbufferA.
14.Applyofchromogen(DAB),10minutes.
15.Washinwater-10minutes.
16.Staininhematoxylinfor5minutes.
17.Washinwater-10minutes.
18.Dehydratethesectionin2changesof96%benzylalcoholfor5minuteseach.
19.Washthesectionin2changesofxylenefor2minuteseach.
20.Mounttheslideforobservation.

*TRIS-EDTABuffer(10mMTRISBase,1mMEDTAsolution,0.05%Tween-20,pH9.0):
TRIS_____________1.21g;
EDTA_____________0.37g;
Distilledwater______1000ml
Mixtodissolvein700mlofdistilledwater.AdjustpHto9.0with1MHClandthenadd0.5mlofTween-20andmixwell.Adjustthefinalvolumeto1literwithdistilledwater.Storethissolutionatroomtemperaturefor3monthsorat+4°Cforlongerstorage.

**CitrateBuffer(10mMCitricAcid,0.05%Tween-20,pH6.0):
Citricacid(anhydrous)______1.92g;
Distilledwater____________1000ml;
Mixtodissolvein700mlofdistilledwater.AdjustpHto6.0with1MNaOHandthenadd0.5mlofTween-20andmixwell.Adjustthefinalvolumeto1literwithdistilledwater.Storethissolutionatroomtemperaturefor3monthsorat+4°Cforlongerstorage.