Description:

This antibody has been successfully used in Western blot procedures to detect µ-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, and rat myoblasts, kidney, liver and spleen.  Immunofluorescence staining of µ-calpain in porcine LLC-PK1 cells with ALX-804-050 results in diffuse cytoplasmic staining.

Product Specification:

Alternative Name:	Calpain I
Clone:	9A4H8D3
Host:	Mouse
Isotype:	IgG1
Immunogen:	Purified bovine µ-calpain.
UniProt ID:	Q27970
Species reactivity:	Human, Mouse, Rat Bovine, Hamster, Porcine, Rabbit
Specificity:	Recognizes an epitope in domain III (aa 465-520) of µ-calpain from several mammalian sources (e.g. human, mouse, rat, pig, bovine, hamster, rabbit).
Crossreactivity:	Does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
Applications:	ICC, IHC, WB
Recommended Dilutions/Conditions:	Immunocytochemistry (1:100) Immunohistochemistry (frozen sections, 1:100) Western Blot (1:2,000) Note: Not recommended for immunoprecipitation.
Application Notes:	Detects a band of ~80kDa by Western blot.
Formulation:	Liquid. Ascites containing 0.05% sodium azide.
Handling:	Avoid freeze/thaw cycles.
Shipping:	Shipped on Blue Ice
Long Term Storage:	-20°C

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