酶科学/E-钙粘蛋白（人）单克隆抗体（SHE78-7）/ALX-804-203-C100/100µ；G
商品编号：
ALX-804-203-C100
品牌：
enzolifesciences
市场价：
¥14500.00
美元价：
8700.00
产品分类：
外泌体研究
公司分类：
exosome_research
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Product Specification:
| Alternative Name: | Uvomorulin, CD324, Epithelial cadherin, Cadherin-1 |
| Clone: | SHE78-7 |
| Host: | Mouse |
| Isotype: | IgG2a |
| Immunogen: | Soluble E-cadherin from human placenta. |
| UniProt ID: | P12830 |
| Source: | Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with soluble E-cadherin from human placenta. |
| Species reactivity: | Human |
| Crossreactivity: | Does not cross-react with rat E-cadherin. |
| Applications: | ELISA, Flow Cytometry, IF, IHC (FS), IHC (PS), WB, FUNC |
| Recommended Dilutions/Conditions: | ELISA (2µg/ml) Flow Cytometry (1µg/ml) Immunofluorescence (1µg/ml) Immunohistochemistry (frozen sections, 2µg/ml) Immunohistochemistry (paraffin sections, 2µg/ml; good results were obtained by incubating with the primary antibody at 4°C overnight) Western Blot (2µg/ml; reducing and non-reducing conditions) Functional Application (1µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Functional Application: inhibits of E-cadherin-dependent cell-cell contact |
| Purity Detail: | Purified from ascites by ammonium sulfate precipitation and anion exchange chromatography. |
| Formulation: | Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA. Contains no preservatives. |
| Reconstitution: | Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl2, 1% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.Note: TBS should be used for dilution, as PBS causes precipitation. |
| Use/Stability: | The stock solution should be stored in aliquotes at -20°C for 1 year or should be stored at 4°C for 6 months after adding 0.1% sodium azide. |
| Handling: | Dilute only prior to immediate use. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
| Shipping: | Blue Ice Not Frozen |
| Short Term Storage: | +4°C |
| Technical Info/Product Notes: | Ca2+ should be contained in the buffers to stabilize cadherin antigen. In case of immunohistochemical staining of paraffin embedded tissues and acetone fixed frozen sections, Ca2+ is not necessary. |
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Company Profile
Name:Enzo Life SciencesCategory:Corporation/Limited Liability CompanyTrade Category: ManufacturerRegistered Time: 1996Registered Capital: Above US$100 MillionLegal Representative:Employees:Address: TaksimWebsite: http://www.enzolifesciences.comProduct/Service: chemicals
Our breadth of scientific expertise coupled with unique technology platforms give us the flexibility to drive innovation throughout the entire research continuum. From life sciences research discovery efforts, through drug development, and into clinical research, our comprehensive range of products are designed to support your goals. When a standard catalog product does not meet your needs, talk to us about custom solutions; we can help
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